Highly motivated, ambitious, driven and talented postdoctoral fellows are sought to join a research program that studies the role of cellular factors on HIV-1 uncoating, reverse transcription and nuclear import in non-dividing cells. Our program has made important contributions to the mechanisms by which HIV-1 productively infect human primary T cells. We have recently discovered the ability of human TRIM5 to block HIV-1 replication, which we plan to investigate in depth using as a model primary human T cells. Moreover, our group has recently demonstrated that HIV-1 nuclear import precedes reverse transcription and uncoating. The individual is expected to join a fast pace moving environment. The laboratory is located in the new Price Center Building, a recently constructed state-of-the-art biomedical research facility centrally located on the campus of the Albert Einstein College of Medicine in New York. Funding is currently available for up to 3 years, with the possibility of further extensions.
This program aims to gain biochemical and cellular biological understanding of the mechanism by which host proteins assist HIV-1 replication. Requirements: a) Recent PhD or MD/Ph.D degree. b) Experience in molecular biology (cloning, shRNA, CRISPR/Cas9, real-time PCR, Western blot), cell biology (microscopy, immunofluorescence and tissue culture), and biochemistry (subcellular fractionation, immunoprecipitations,etc). c) experience working with human primary T cells and mouse models.
Applicants must have at least 1 paper in a peered reviewed journal. Please send a CV, and names for up to three references by email to Felipe Diaz-Griffero (email@example.com).
Selyutina, A., Persaud, M., Lee, K., KewalRamani, V., and Diaz-Griffero, F. (2020a). Nuclear Import of the HIV-1 Core Precedes Reverse Transcription and Uncoating. Cell Rep 32, 108201.
Selyutina, A., Persaud, M., Simons, L.M., Bulnes-Ramos, A., Buffone, C., Martinez-Lopez, A., Scoca, V., Di Nunzio, F., Hiatt, J., Marson, A., et al. (2020b). Cyclophilin A Prevents HIV-1 Restriction in Lymphocytes by Blocking Human TRIM5alpha Binding to the Viral Core. Cell Rep 30, 3766-3777 e3766.
Yu, C.H., Bhattacharya, A., Persaud, M., Taylor, A.B., Wang, Z., Bulnes-Ramos, A., Xu, J., Selyutina, A., Martinez-Lopez, A., Cano, K., et al. (2021). Nucleic acid binding by SAMHD1 contributes to the antiretroviral activity and is enhanced by the GpsN modification. Nature communications 12, 731.