Postdoctoral Fellowship, in vivo single-cell sequencing of virus infection

(Ref#: 0001)

The National Institute of Allergy and Infectious Diseases (NIAID)

Categories:
  • Post Doctoral Position - Academic
  • Post Doctoral Position - Industry
Job Type/Arrangement:
  • Full Time
  • Fellowship
Description:

The Quantitative Virology and Evolution Unit, led by Patrick Dolan, Ph.D., within the Laboratory of Viral Diseases is seeking a postdoctoral fellow to develop projects in within-host evolution and pathogenesis of enteroviruses and other RNA viruses. The candidate will develop an independent project focused on the dynamics of host responses and viral evolution with access to a wide range of techniques and resources to pursue these questions. 

Responsibilities: 

  • Develop independent projects focused on characterizing the histopathological and transcriptional phenotypes of host cells through single-cell sequencing, spatial sequencing, virus population sequencing, and microscopy in tissues from mice infected with enteroviruses or other RNA viruses. 
  • Perform molecular biology and virology experiments in support of animal experiments. 
  • Process and analyze single-cell sequencing data using various pipelines (e.g. kallisto, CellRanger).
  • Develop methods to sequence individual viral genome sequences from mouse tissues. 
  • Work with animal facility and QVEU staff to plan experiments and effectively manage mouse colony. 

Qualifications: 

  • A Ph.D., D.V.M., D.O., or M.D. degree.
  • Strong record of research experience in virology, immunology, evolutionary biology, and/or cell biology. 

Applicants with 2 of 5 should consider themselves well qualified:

  • 2-3 years experience working with rodent model systems. 
  • Mouse infection, dissection, and virus quantification from tissues. 
  • Sequencing experience (Illumina, Nanopore, or PacBio).
  • Computational experience - proficiency with R (esp. tidyverse), python, high-performance computing (e.g. Slurm, PBS), and/or sequencing pipelines (kallisto, bowtie, minimap). 
  • Experience with single-cell sequencing approaches.

Please note: The QVEU seeks to provide a supportive, equitable, safe, and fun environment to explore emerging questions in virology. Those from historically marginalized or under-represented backgrounds are especially encouraged to apply. U.S. citizenship is not required (visa requirements apply)

Interested applicants should e-mail a CV and cover letter as a single PDF to Patrick.Dolan@nih.gov

Education Requirement:
Ph.D., DVM, MD, DO
External job URL:
https://qveu.github.io/QVEU/
Salary:
60000+ depending on experience
Notes:

The QVEU is located within the Laboratory of Viral Diseases (LVD)  within the National Institute of Allergy and Infectious Diseases (NIAID) on the main NIH campus in Bethesda, Maryland. LVD is composed of nine independent investigators who seek fundamental insights into the complex biology of human viruses. Current research covers a broad array of viral pathogens (HIV, poxviruses, herpesviruses, papillomaviruses, influenza viruses, and enteroviruses, alphaviruses, and flaviviruses) and explores virtually all facets of viral biology, including virus entry, regulation of gene expression, mechanisms of genome replication, virion morphogenesis, latency-persistence, pathogenesis, immunity, and evolution. LVD investigators also apply their expertise to develop viral vaccines and therapeutics.

Location:
Main Campus, Bethesda, MD
Laboratory of Viral Diseases
Building 33, Rm. 1E13C.4
33 North Drive, MSC 3210
Bethesda , MD 20892-3210
US
Contact:
Patrick Dolan, Ph.D.